Checklist for reporting a study using laboratory animals

This checklist is relevant to research performed on animals in a laboratory setting, and is based on the ARRIVE guidelines.  Read more

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Complete this checklist by entering the page numbers from your manuscript where readers will find each of the items listed below.

Your article may not currently address all the items on the checklist. Please modify your text to include the missing information. If you are certain that an item does not apply, please write "n/a" and provide a short explanation.

Download your completed checklist and include it as an extra file when you submit to a journal.

Essential 10


Study design*

Give details of the groups being compared, including control groups. If no control group has been used, the rationale should be stated. Read more


Study design*

Give details of the experimental unit (e.g., a single animal, litter, or cage of animals). Read more


Sample size*

Specify the exact number of experimental units allocated to each group, and the total number in each experiment. Also indicate the total number of animals used. Read more


Sample size*

Explain how the sample size was decided. Provide details of any a priori sample size calculation, if done. Read more


Inclusion and exclusion criteria*

Describe any criteria used for including or excluding animals (or experimental units) during the experiment, and data points during the analysis. Specify if these criteria were established a priori. If no criteria were set, state this explicitly. Read more


Inclusion and exclusion criteria*

For each experimental group, report any animals, experimental units, or data points not included in the analysis and explain why. If there were no exclusions, state so. Read more


Inclusion and exclusion criteria*

For each analysis, report the exact value of n in each experimental group. Read more



State whether randomisation was used to allocate experimental units to control and treatment groups. If done, provide the method used to generate the randomisation sequence. Read more



Describe the strategy used to minimise potential confounders such as the order of treatments and measurements, or animal/cage location. If confounders were not controlled, state this explicitly. Read more



Describe who was aware of the group allocation at the different stages of the experiment (during the allocation, the conduct of the experiment, the outcome assessment, and the data analysis). Read more


Outcome measures*

Clearly define all outcome measures assessed (e.g., cell death, molecular markers, or behavioural changes). Read more


Outcome measures*

For hypothesis-testing studies, specify the primary outcome measure, i.e., the outcome measure that was used to determine the sample size. Read more


Statistical methods*

Provide details of the statistical methods used for each analysis, including software used. Read more


Statistical methods*

Describe any methods used to assess whether the data met the assumptions of the statistical approach, and what was done if the assumptions were not met. Read more


Experimental animals*

Provide species-appropriate details of the animals used, including species, strain and substrain, sex, age or developmental stage, and, if relevant, weight. Read more


Experimental animals*

Provide further relevant information on the provenance of animals, health/immune status, genetic modification status, genotype, and any previous procedures. Read more


Experimental procedures*

For each experimental group, including controls, describe the procedures in enough detail to allow others to replicate what was done, how it was done, and what was used. Read more


Experimental procedures*

Timing and frequency of procedures. Read more


Experimental procedures*

Where procedures were carried out (including detail of any acclimatisation periods). Read more


Experimental procedures*

Rationale for procedures. Read more



For each experiment conducted, including independent replications, report summary/descriptive statistics for each experimental group, with a measure of variability where applicable (e.g., mean and SD, or median and range). Read more



If applicable, for each experiment conducted, including independent replications, report the effect size with a confidence interval. Read more

Recommended set



Provide an accurate summary of the research objectives, animal species, strain and sex, key methods, principal findings, and study conclusions. Read more



Include sufficient scientific background to understand the rationale and context for the study, and explain the experimental approach. Read more



Explain how the animal species and model used address the scientific objectives and, where appropriate, the relevance to human biology. Read more



Clearly describe the research question, research objectives and, where appropriate, specific hypotheses being tested. Read more


Ethical statement

Provide the name of the ethical review committee or equivalent that has approved the use of animals in this study and any relevant licence or protocol numbers (if applicable). If ethical approval was not sought or granted, provide a justification. Read more


Housing and husbandry

Provide details of housing and husbandry conditions, including any environmental enrichment. Read more


Animal care and monitoring

Describe any interventions or steps taken in the experimental protocols to reduce pain, suffering, and distress. Read more


Animal care and monitoring

Report any expected or unexpected adverse events. Read more


Animal care and monitoring

Describe the humane endpoints established for the study, the signs that were monitored, and the frequency of monitoring. If the study did not set humane endpoints, state this. Read more


Interpretation/scientific implications

Interpret the results, taking into account the study objectives and hypotheses, current theory, and other relevant studies in the literature. Read more


Interpretation/scientific implications

Comment on the study limitations, including potential sources of bias, limitations of the animal model, and imprecision associated with the results. Read more



Comment on whether, and how, the findings of this study are likely to generalise to other species or experimental conditions, including any relevance to human biology (where appropriate). Read more


Protocol registration

Provide a statement indicating whether a protocol (including the research question, key design features, and analysis plan) was prepared before the study, and if and where this protocol was registered. Read more


Data access

Provide a statement describing if and where study data are available. Read more


Declaration of interests

Declare any potential conflicts of interest, including financial and nonfinancial. If none exist, this should be stated. Read more


Declaration of interests

List all funding sources (including grant identifier) and the role of the funder(s) in the design, analysis, and reporting of the study. Read more

To acknowledge this checklist in your methods, please state "We used the ARRIVE checklist when writing our report [citation]". Then cite this checklist as Percie du Sert N, Hurst V, Ahluwalia A, Alam S, Avey MT, Baker M, Browne WJ, Clark A, Cuthill IC, Dirnagl U, Emerson M, Garner P, Holgate ST, Howells DW, Karp NA, Lazic SE, Lidster K, MacCallum CJ, Macleod M, Pearl EJ, Petersen O, Rawle F, Peynolds P, Rooney K, Sena ES, Silberberg SD, Steckler T and Wurbel H. The ARRIVE Guidelines 2.0: updated guidelines for reporting animal research..

The ARRIVE checklist is distributed under the terms of the Creative Commons Attribution License CC-BY